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Fascin Monoclonal antibody
Fascin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
1B5G3
Cat no : 66321-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | SH-SY5Y cells, HeLa cells, K-562 cells |
Positive IHC detected in | human tonsillitis tissue, human pancreas cancer tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human tonsillitis tissue |
Positive IF/ICC detected in | SH-SY5Y cells |
Positive FC (Intra) detected in | SH-SY5Y cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 2 publications below |
IHC | See 1 publications below |
IF | See 2 publications below |
Product Information
66321-1-Ig targets Fascin in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | Fascin fusion protein Ag6074 相同性解析による交差性が予測される生物種 |
Full Name | fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus) |
Calculated molecular weight | 55 kDa |
Observed molecular weight | 55 kDa |
GenBank accession number | BC000521 |
Gene symbol | Fascin |
Gene ID (NCBI) | 6624 |
RRID | AB_2881701 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Fascin antibody 66321-1-Ig | Download protocol |
IHC protocol for Fascin antibody 66321-1-Ig | Download protocol |
IF protocol for Fascin antibody 66321-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Prolif Formin homology domains of Daam1 bind to Fascin and collaboratively promote pseudopodia formation and cell migration in breast cancer.
| ||
Cytoskeleton (Hoboken) The myosin regulatory light chain Myl5 localizes to mitotic spindle poles and is required for proper cell division. | ||
MicroPubl Biol The actin bundling protein Fascin is important for proper early development in Strongylocentrotus purpuratus embryos |