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CoraLite® Plus 647-conjugated Fascin Monoclonal antibody
Fascin Monoclonal Antibody for IF-P, FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
IF-P, FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
1B5G3
Cat no : CL647-66321
Synonyms
Validation Data Gallery
Tested Applications
Positive IF-P detected in | human tonsillitis tissue |
Positive FC (Intra) detected in | HeLa cells |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL647-66321 targets Fascin in IF-P, FC (Intra) applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | Fascin fusion protein Ag6074 相同性解析による交差性が予測される生物種 |
Full Name | fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus) |
Calculated molecular weight | 55 kDa |
Observed molecular weight | 55 kDa |
GenBank accession number | BC000521 |
Gene symbol | Fascin |
Gene ID (NCBI) | 6624 |
RRID | AB_2920280 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 647 Fascin antibody CL647-66321 | Download protocol |
FC protocol for CL Plus 647 Fascin antibody CL647-66321 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |