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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Filamin B Polyclonal antibody (20685-1-AP)

    WB analysis using 20685-1-AP (same clone as 20685-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20685-1-AP (Filamin B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20685-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using Filamin B Polyclonal antibody (20685-1-AP)

    WB analysis of NIH/3T3 using 20685-1-AP (same clone as 20685-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20685-1-AP (FLNB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20685-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using Filamin B Polyclonal antibody (20685-1-AP)

    WB analysis of U2OS using 20685-1-AP (same clone as 20685-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 20685-1-AP (FLNB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20685-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Filamin B Polyclonal antibody (20685-1-AP)

    IHC staining of human liver cancer using 20685-1-AP (same clone as 20685-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20685-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Filamin B Polyclonal antibody (20685-1-AP)

    IHC staining of human liver cancer using 20685-1-AP (same clone as 20685-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20685-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Filamin B Polyclonal antibody (20685-1-AP)

    IHC staining of human colon using 20685-1-AP (same clone as 20685-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20685-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Filamin B Polyclonal antibody (20685-1-AP)

    IHC staining of human colon using 20685-1-AP (same clone as 20685-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 20685-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Filamin B Polyclonal antibody (20685-1-AP)

    IHC staining of human colon using 20685-1-AP (same clone as 20685-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 20685-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Filamin B Polyclonal antibody (20685-1-AP)

    IHC staining of human colon using 20685-1-AP (same clone as 20685-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 20685-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Filamin B Polyclonal antibody (20685-1-AP)

    IHC staining of human colon using 20685-1-AP (same clone as 20685-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 20685-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Filamin B Polyclonal antibody (20685-1-AP)

    IF Staining of HepG2 using 20685-1-AP (same clone as 20685-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FLNB antibody (20685-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 20685-1-PBS in a different storage buffer formulation.

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Filamin B Polyclonal antibody, PBS Only

Filamin B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20685-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

FLNB, FilaminB, Filamin-3, Beta-filamin, Actin-binding-like protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20685-1-PBS targets Filamin B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name filamin B, beta (actin binding protein 278)
Calculated molecular weight 278 kDa
Observed molecular weight 250-278 kDa
GenBank accession numberNM_001457
Gene Symbol FLNB
Gene ID (NCBI) 2317
RRIDAB_10863658
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75369
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

FLNB, also named as FLN1L, FLN3, TABP, TAP and Fh1, belongs to the filamin family. FLNB connects cell membrane constituents to the actin cytoskeleton. It may promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. It is an anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA, FLNB may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. The antibody is specific to FLNB. It can recognize isoform1,2,3,6,7 of FLNB. It has no cross reaction to FLNA and FLNC.