HRP-conjugated DYKDDDDK Tag Monoclonal antibody (Binds to FLAG® tag epitope)

DYKDDDDK Tag Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2b

Reactivity

recombinant protein

Applications

WB, CoIP, ELISA

Conjugate

HRP

CloneNo.

8H6A10

Cat no : HRP-66008

Synonyms

DDDDK tag, DYKDDDDK, DYKDDDDK Tag, HRP conjugated DDDDK tag, HRP-66008, HRP-DDDDK tag


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Tested Applications

Positive WB detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:10000-1:100000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

HRP-66008 targets DYKDDDDK Tag in WB, CoIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name DYKDDDDK Tag
Gene symbol
Gene ID (NCBI)
RRIDAB_2883835
Conjugate HRP
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DYKDDDDK tag, also known as DDK tag, is a hydrophilic tag for recombinant protein technology with the sequence DYKDYKDDDDK. The structure of DYKDDDDK tag has been optimized for compatibility with the proteins it is attached to and unlikely to denature or inactivated them. For its high hydrophilic nature, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag more likely to be accessible to antibodies. This product is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, no matter it is/they are located at N-terminal, C-terminal or at internal regions. The binding of this product with DYKDDDDK tagged fusion protein does not depend on calcium ion. The clone of this monoclonal antibody hybridoma is 1E7B4, which is different from any other vendors. This antibody is HRP conjugated DYKDDDDK Tag antibody. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Protocols

Product Specific Protocols
WB protocol for HRP DYKDDDDK Tag antibody HRP-66008Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Nat Commun

A lung-selective delivery of mRNA encoding broadly neutralizing antibody against SARS-CoV-2 infection

Authors - Wanbo Tai
WB

Nat Commun

FAM222A encodes a protein which accumulates in plaques in Alzheimer's disease.

Authors - Tingxiang Yan

PLoS Pathog

Vibrio splendidus infection promotes circRNA-FGL1-regulated coelomocyte apoptosis via competitive binding to Myc with the deubiquitinase OTUB1 in Apostichopus japonicus

Authors - Ming Guo
WB

Int Immunopharmacol

ZAP facilitates NLRP3 inflammasome activation via promoting the oligomerization of NLRP3

Authors - Danhui Qin
WB

Liver Int

The DEAD-box helicase DDX3x ameliorates non-alcoholic fatty liver disease via mTORC1 signalling pathway.

Authors - Peihao Liu

Biomed Pharmacother

PMID: 32036219

Authors - Yu Chen