Validation Data Gallery
Product Information
| Product name | FlexAble 2.0 CoraLite® Plus 488 Antibody Labeling Kit for Rabbit IgG |
| Assay type | Antibody labeling |
| Tested applications | IF, FC, WB |
| Species Reactivity | Rabbit IgG |
| Antibody amount per labeling reaction | 0.5 µg antibody |
| Conjugate | CoraLite® Plus 488 |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Kit Components
| Component | 10rxns | 50rxns | 200rxns |
|---|---|---|---|
| FlexBuffer | 100ul | 500ul | 2000ul |
| FlexLinker 2.0 CoraLite® Plus 488 for Rabbit IgG | 10ul | 50ul | 200ul |
| FlexQuencher for Rabbit IgG | 20ul | 100ul | 400ul |
Ship Condition
shipped on blue iceStorage Condition
Store for 1 year at -20°C or for 6 months at +4°C upon receipt. Avoid exposure to lightFAQ
Q: I have limited sample diluent. How should I handle samples requiring high dilution factors?
A: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Q: What is the difference between FlexAble and FlexAble 2.0 products?
A: FlexAble 2.0 has been improved to allow for more dyes per FlexLinker than the previous version of the kit. This results in an increased signal per antibody.
Q: What are the FlexLinker, FlexQuencher and FlexBuffer?
A: The FlexLinker is a small polypeptide to which dyes are covalently conjugated that can label unconjugated primary antibodies. The FlexQuencher is an Fc-containing fragment that neutralizes the excess FlexLinker. The FlexBuffer is a PBS-based buffer.
Q: What is the largest quantity I can label?
A: With a standard kit size (50 reactions), you can label 25 µg of one antibody or up to 50 different antibodies. You can easily scale up the antibody amount per labeling approach.
Q: What is the lowest concentration of my primary antibody that I can use?
A: Our protocol uses 0.5 µg of primary antibody in 7 µL, which ends up at 0.07 mg/mL. If the concentration of your antibody is lower, you can also use a larger volume than 7 µL.
Q: Can I label primary antibodies stored in BSA, glycerol, Tris buffer and/or preservatives?
A: Yes, FlexAble Antibody Labeling Kits have been validated with carriers and amine buffers. Neither BSA nor amine buffers, in any chosen concentration, interfere with the labeling. 50% glycerol as well as preservatives like sodium azide are also compatible with the kit.
Q: How many different primary antibodies can I label with one kit?
A: You can label up to 50 different antibodies with our FlexAble 50 rxn Kit, and up to 10 antibodies with our FlexAble 10 rxn Kit.
Q: Will I observe cross-reactivity/leaking when I use two FlexAble-labeled antibodies from the same species during multiplexing?
A: FlexAble labels primary antibodies with a high affinity FlexLinker. Dissociation of FlexLinker from one antibody and association to another antibody is rare. If you observe leaking, we recommend adding more FlexQuencher to remove unbound FlexLinker, or you can try sequential staining of the labeled antibodies.
Documentation
| SDS |
|---|
| KFA501-SDS-FlexAble 2.0 CoraLite Plus 488 Antibody Labeling Kit |
| Protocol |
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| Protocol-FlexAble and FlexAble 2.0 Antibody Labeling Kits |
| Datasheet |
|---|
| FlexAble 2.0 CoraLite® Plus 488 Antibody Labeling Kit for Rabbit IgG Datasheet |
| White papers |
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| Efficient Hybridoma Screening with FlexAble Antibody Labeling Kits |
| Customer testimonials |
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| Léa Pechtimaldjian on Advancing 3D Vascular Imaging with FlexAble Technology |
Publications
| Application | Title |
|---|---|
Cell Mol Immunol Oncolytic virus M1 reinvigorates CD8(+) T-cell immunity against glioblastoma through B-cell-dependent antigen cross-presentation in the spleen. | |
Sci Adv The chloride intracellular channel 1 (CLIC1) is essential for microglial morphodynamics and neuroinflammation | |
Invest Ophthalmol Vis Sci Quantitative Changes in the Proteome of Chronically Inflamed Lacrimal Glands From a Sjögren's Disease Animal Model | |
Redox Biol Modification of the dermal matrix by senescence associated lipids and its functional consequence. | |
Cell Rep PATZ1 condensation adjacent to PML nuclear bodies suppresses HBoV transcription as an intrinsic antiviral defense. | |