Validation Data Gallery
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Tested Applications
| Positive WB detected in | U-87 MG cells, LNCaP cells, Raji cells, mouse heart tissue, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83598-4-RR targets Flotillin 1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Flotillin 1 fusion protein Ag7902 相同性解析による交差性が予測される生物種 |
| Full Name | flotillin 1 |
| Calculated molecular weight | 47 kDa |
| Observed molecular weight | 47 kDa |
| GenBank accession number | BC001146 |
| Gene Symbol | Flotillin 1 |
| Gene ID (NCBI) | 10211 |
| RRID | AB_3671213 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | O75955 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins commonly used as lipid raft markers. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Flotillin 1 antibody 83598-4-RR | Download protocol |
| IHC protocol for Flotillin 1 antibody 83598-4-RR | Download protocol |
| IF protocol for Flotillin 1 antibody 83598-4-RR | Download protocol |
| IP protocol for Flotillin 1 antibody 83598-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |