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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Flotillin 1 Recombinant antibody (83598-4-RR)

    WB analysis using 83598-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83598-4-RR (Flotillin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using Flotillin 1 Recombinant antibody (83598-4-RR)

    IP experiment of mouse brain using 83598-4-RR

    IP result of anti-Flotillin 1 (IP:83598-4-RR, 4ug; Detection:83598-4-RR 1:3000) with mouse brain tissue lysate 2400 ug.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Flotillin 1 Recombinant antibody (83598-4-RR)

    IHC staining of human stomach cancer using 83598-4-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83598-4-RR (Flotillin 1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Flotillin 1 Recombinant antibody (83598-4-RR)

    IHC staining of human stomach cancer using 83598-4-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83598-4-RR (Flotillin 1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Flotillin 1 Recombinant antibody (83598-4-RR)

    IF Staining of HeLa using 83598-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Flotillin 1 antibody (83598-4-RR, Clone: 240582G6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83598-4-RR

    Affinity and Kinetic Characterization of 83598-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83598-4-RR against Human Flotillin 1 were performed. The affinity constant is 30.5 nM.

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Flotillin 1 Recombinant antibody

Flotillin 1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 83598-4-RR
Clone No.240582G6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

FLOT1, 240582G6, Flotillin-1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU-87 MG cells, LNCaP cells, Raji cells, mouse heart tissue, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83598-4-RR targets Flotillin 1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Flotillin 1 fusion protein Ag7902 相同性解析による交差性が予測される生物種
Full Name flotillin 1
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC001146
Gene Symbol Flotillin 1
Gene ID (NCBI) 10211
RRIDAB_3671213
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO75955
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins commonly used as lipid raft markers. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.

Protocols

Product Specific Protocols
WB protocol for Flotillin 1 antibody 83598-4-RRDownload protocol
IHC protocol for Flotillin 1 antibody 83598-4-RRDownload protocol
IF protocol for Flotillin 1 antibody 83598-4-RRDownload protocol
IP protocol for Flotillin 1 antibody 83598-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols