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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Flotillin 1 Recombinant antibody

Flotillin 1 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240582G6

Cat no : CL488-83598-4

Synonyms

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated Flotillin 1 Recombin (CL488-83598-4)

    IF Staining of HeLa using CL488-83598-4

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 Flotillin 1 antibody (CL488-83598-4, Clone: 240582G6 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-83598-4 is Flotillin 1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Flotillin 1 fusion protein Ag7902 相同性解析による交差性が予測される生物種
Full Name flotillin 1
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC001146
Gene symbol Flotillin 1
Gene ID (NCBI) 10211
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins commonly used as lipid raft markers. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Flotillin 1 antibody CL488-83598-4Download protocol
Standard Protocols
Click here to view our Standard Protocols