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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Follistatin Monoclonal antibody (60060-1-Ig)

    WB analysis using 60060-1-Ig (same clone as 60060-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60060-1-Ig (Follistatin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60060-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using Follistatin Monoclonal antibody (60060-1-Ig)

    WB analysis of Raji using 60060-1-Ig (same clone as 60060-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 60060-1-Ig (Follistatin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60060-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Follistatin Monoclonal antibody (60060-1-Ig)

    WB analysis using 60060-1-Ig (same clone as 60060-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60060-1-Ig (Follistatin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60060-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using Follistatin Monoclonal antibody (60060-1-Ig)

    WB analysis of HepG2 using 60060-1-Ig (same clone as 60060-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60060-1-Ig (Follistatin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60060-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Follistatin Monoclonal antibody (60060-1-Ig)

    IHC staining of human ovary tumor using 60060-1-Ig (same clone as 60060-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60060-1-Ig (Follistatin antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60060-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Follistatin Monoclonal antibody (60060-1-Ig)

    IHC staining of human ovary tumor using 60060-1-Ig (same clone as 60060-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60060-1-Ig (Follistatin antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60060-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Follistatin Monoclonal antibody (60060-1-Ig)

    IF Staining of HeLa using 60060-1-Ig (same clone as 60060-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Follistatin antibody (60060-1-Ig, Clone: 4E9B10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60060-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Follistatin Monoclonal antibody, PBS Only

Follistatin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 60060-1-PBS
Clone No.4E9B10

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

FST, FS, Activin-binding protein, Activin binding protein, 4E9B10

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60060-1-PBS targets Follistatin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Follistatin fusion protein Ag0775 相同性解析による交差性が予測される生物種
Full Name follistatin
Calculated molecular weight 33 aa, 7 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC004107
Gene Symbol Follistatin
Gene ID (NCBI) 10468
RRIDAB_2106706
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP19883
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Follistatin (FST) is a member of the tissue growth factor β family and is a secreted glycoprotein that antagonizes many members of the family, including activin A, growth differentiation factor11 , and myostatin. It binds activin A with high affinity and whose expression can be induced by activin A and several other pro-inflammatory cytokines. Activin A-follistatin complexes are biologically inactive and bind to cell surface heparan sulphate-containing proteoglycans for internalisation and degradation. It has also been found to play a significant role in the management of skeletal muscle size and mass. It also has important roles in early embryonic development, differentiation of ovarian granulosa cells, liver fibrosis and polycystic ovarian syndrome. FS 288 glycosylation results in a major species of 35 kDa and a less abundant 40 kDa and minor products of 40 and 42 kDa (PMID: 9785474). Three typical follistatin bands are at 32, 35, and 39 kDa (PMID: 2036994).