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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Frizzled 9 Monoclonal antibody

Frizzled 9 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G9G4

Cat no : CL488-67023

Synonyms

FZD9, FZD3, Fz-9, Fz 9, Frizzled-9

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated Frizzled 9 Monoclona (CL488-67023)

    FC experiment of HeLa using CL488-67023

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Frizzled 9 (CL488-67023, Clone:1G9G4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67023 targets Frizzled 9 in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Frizzled 9 fusion protein Ag4971 相同性解析による交差性が予測される生物種
Full Name frizzled homolog 9 (Drosophila)
Calculated molecular weight 591 aa, 64 kDa
Observed molecular weight 64-70 kDa
GenBank accession numberBC026333
Gene symbol Frizzled 9
Gene ID (NCBI) 8326
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 Frizzled 9 antibody CL488-67023Download protocol
Standard Protocols
Click here to view our Standard Protocols