Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 13057-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13057-2-AP (G3BP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 13057-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13057-2-AP (G3BP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 13057-2-AP
WB result of G3BP1 antibody (13057-2-AP; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-G3BP1 transfected HEK-293 cells.
WB analysis using 13057-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13057-2-AP (G3BP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 13057-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13057-2-AP (G3BP1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis using 13057-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13057-2-AP (G3BP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 13057-2-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 13057-2-AP (G3BP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 13057-2-AP
IP result of anti-G3BP1 (IP:13057-2-AP, 4ug; Detection:13057-2-AP 1:1000) with HEK-293 cells lysate 1040 ug.
IHC staining of human lung cancer using 13057-2-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13057-2-AP (G3BP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 13057-2-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13057-2-AP (G3BP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 13057-2-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13057-2-AP (G3BP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 13057-2-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13057-2-AP (G3BP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 13057-2-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13057-2-AP (G3BP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 13057-2-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13057-2-AP (G3BP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 13057-2-AP
Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP1 antibody (13057-2-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa using 13057-2-AP
Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP1 antibody (13057-2-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of HeLa using 13057-2-AP
1x10^6 HeLa cells were intracellularly stained with 0.25 ug G3BP1 Polyclonal antibody (13057-2-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

G3BP1 Polyclonal antibody

G3BP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 13057-2-AP

Publications(216)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Synonyms

ATP-dependent DNA helicase VIII, EC:3.6.4.12, G3BP, G3BP 1, G3BP-1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®555 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (216)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	C6 cells, HEK-293 cells, human brain tissue, Neuro-2a cells, Jurkat cells, MCF-7 cells, HeLa cells, mouse kidney tissue, rat kidney tissue, mouse brain tissue, rat brain tissue
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human lung cancer tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	sodium arsenite treated HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 11 publications below
WB	See 107 publications below
IHC	See 13 publications below
IF	See 156 publications below
IP	See 20 publications below
CoIP	See 8 publications below
RIP	See 4 publications below

Product Information

13057-2-AP targets G3BP1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, monkey, chicken, zebrafish, drosophila melanogaster (fruit fly)
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3728 Product name: Recombinant human G3BP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 167-466 aa of BC006997 Sequence: PDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEPEPEQEPVSEIQEEKPEPVLEETAPEDAQKSSSPAPADIAQTVQEDLRTFSWASVTSKNLPPSGAVPVTGIPPHVVKVPASQPRPESKPESQIPPQRPQRDQRVREQRINIPPQRGPRPIREAGEQGDIEPRRMVRHPDSHQLFIGNLPHEVDKSELKDFFQSYGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLSNRPIMFRGEVRLNVEEKKTRAAREGDRRDNRLRGPGGPRGGLGGGMRGPPRGGMVQKPGFGVGRGLAPRQ 相同性解析による交差性が予測される生物種
Full Name	GTPase activating protein (SH3 domain) binding protein 1
Calculated molecular weight	466 aa, 52 kDa
Observed molecular weight	68 kDa
GenBank accession number	BC006997
Gene Symbol	G3BP1
Gene ID (NCBI)	10146
RRID	AB_2232034
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13283
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.

Protocols

Product Specific Protocols
IP protocol for G3BP1 antibody 13057-2-AP	Download protocol
WB protocol for G3BP1 antibody 13057-2-AP	Download protocol
FC protocol for G3BP1 antibody 13057-2-AP	Download protocol
IF protocol for G3BP1 antibody 13057-2-AP	Download protocol
IHC protocol for G3BP1 antibody 13057-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse,human,chicken	IF,CoIP	Cell Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules Authors - Qinqin Cui View Article
human	IF	Nature m6A enhances the phase separation potential of mRNA. Authors - Ryan J Ries View Article
human	IP,IF	Science Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner. Authors - Youngdae Gwon View Article
human	IF	Cell ELAVL4, splicing, and glutamatergic dysfunction precede neuron loss in MAPT mutation cerebral organoids. Authors - Kathryn R Bowles View Article
human	IF	Cell RNA Granules Hitchhike on Lysosomes for Long-Distance Transport, Using Annexin A11 as a Molecular Tether. Authors - Ya-Cheng Liao View Article
human	IF	Cell Phase Separation of FUS Is Suppressed by Its Nuclear Import Receptor and Arginine Methylation. Authors - Mario Hofweber View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 13057-2-AP

WB analysis using 13057-2-AP

WB analysis of HEK-293 using 13057-2-AP

WB analysis using 13057-2-AP

WB analysis using 13057-2-AP

WB analysis using 13057-2-AP

WB analysis of human brain using 13057-2-AP

IP experiment of HEK-293 using 13057-2-AP

IHC staining of human lung cancer using 13057-2-AP

IHC staining of human lung cancer using 13057-2-AP

IHC staining of human lung cancer using 13057-2-AP

IHC staining of human lung cancer using 13057-2-AP

IHC staining of human colon cancer using 13057-2-AP

IHC staining of human colon cancer using 13057-2-AP

IF Staining of HeLa using 13057-2-AP

IF Staining of HeLa using 13057-2-AP

FC experiment of HeLa using 13057-2-AP

G3BP1 Polyclonal antibody

G3BP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules

m6A enhances the phase separation potential of mRNA.

Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner.

ELAVL4, splicing, and glutamatergic dysfunction precede neuron loss in MAPT mutation cerebral organoids.

RNA Granules Hitchhike on Lysosomes for Long-Distance Transport, Using Annexin A11 as a Molecular Tether.

Phase Separation of FUS Is Suppressed by Its Nuclear Import Receptor and Arginine Methylation.