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CoraLite®555-conjugated G3BP1 Polyclonal antibody
G3BP1 Polyclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite®555 Fluorescent Dye
Cat no : CL555-13057
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | sodium arsenite treated HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL555-13057 targets G3BP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, rat, mouse samples.
| Tested Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | G3BP1 fusion protein Ag3728 相同性解析による交差性が予測される生物種 |
| Full Name | GTPase activating protein (SH3 domain) binding protein 1 |
| Calculated molecular weight | 466 aa, 52 kDa |
| Observed molecular weight | 55-60 kDa |
| GenBank accession number | BC006997 |
| Gene symbol | G3BP1 |
| Gene ID (NCBI) | 10146 |
| RRID | AB_2919614 |
| Conjugate | CoraLite®555 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 557 nm / 570 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL555 G3BP1 antibody CL555-13057 | Download protocol |
| FC protocol for CL555 G3BP1 antibody CL555-13057 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
