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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

G3BP1 Recombinant antibody

G3BP1 Recombinant Antibody for IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

7F14

Cat no : 80777-1-RR

Synonyms

G3BP-1, G3BP 1, G3BP, EC:3.6.4.12, ATP-dependent DNA helicase VIII

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using G3BP1 Recombinant antibody (80777-1-RR)

    IHC staining of mouse brain using 80777-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80777-1-RR (G3BP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using G3BP1 Recombinant antibody (80777-1-RR)

    IHC staining of mouse brain using 80777-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80777-1-RR (G3BP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using G3BP1 Recombinant antibody (80777-1-RR)

    IF Staining of mouse brain using 80777-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using G3BP1 antibody (80777-1-RR, Clone: 7F14 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using G3BP1 Recombinant antibody (80777-1-RR)

    IF Staining of HeLa using 80777-1-RR

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP1 antibody (80777-1-RR, Clone: 7F14 ) at dilution of 1:1400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 80777-1-RR

    Affinity and Kinetic Characterization of 80777-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80777-1-RR against Human G3BP1 were performed. The affinity constant is 7.07 nM.

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Tested Applications

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected insodium arsenite treated HeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80777-1-RR targets G3BP1 in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen G3BP1 fusion protein Ag3728 相同性解析による交差性が予測される生物種
Full Name GTPase activating protein (SH3 domain) binding protein 1
Calculated molecular weight 466 aa, 52 kDa
GenBank accession numberBC006997
Gene symbol G3BP1
Gene ID (NCBI) 10146
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.

Protocols

Product Specific Protocols
IHC protocol for G3BP1 antibody 80777-1-RRDownload protocol
IF protocol for G3BP1 antibody 80777-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols