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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®555-conjugated G3BP1 Recombinant antibody

G3BP1 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

7F14

Cat no : CL555-80777

Synonyms

G3BP, G3BP 1, G3BP1, HDH VIII

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®555-conjugated G3BP1 Recombinant antibody (CL555-80777)

    IF Staining of HeLa using CL555-80777

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CoraLite®555 G3BP1 antibody (CL555-80777, Clone: 7F14 ) at dilution of 1:200, CL488-Phalloidin (green).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®555-conjugated G3BP1 Recombinant antibody (CL555-80777)

    IF Staining of HeLa using CL555-80777

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CoraLite®555 G3BP1 antibody (CL555-80777, Clone: 7F14 ) at dilution of 1:200, CL488-Phalloidin (green).

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Tested Applications

Positive IF/ICC detected insodium arsenite treated HeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-80777 targets G3BP1 in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen G3BP1 fusion protein Ag3728 相同性解析による交差性が予測される生物種
Full Name GTPase activating protein (SH3 domain) binding protein 1
Calculated molecular weight 466 aa, 52 kDa
GenBank accession numberBC006997
Gene symbol G3BP1
Gene ID (NCBI) 10146
RRIDAB_2919712
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.

Protocols

Product Specific Protocols
IF protocol for CL555 G3BP1 antibody CL555-80777Download protocol
Standard Protocols
Click here to view our Standard Protocols