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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using G3BP2 Recombinant antibody (82080-4-RR)

    WB analysis using 82080-4-RR (same clone as 82080-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82080-4-RR (G3BP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82080-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00090-3

    Sandwich ELISA standard curve of MP00090-3

    Sandwich ELISA standard curve of MP00090-3, Human G3BP2 Recombinant Matched Antibody Pair - PBS only. 82080-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag9355. 82080-2-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

  • Sample test of MP00090-3

    Sample test of MP00090-3

    The mean G3BP2 concentration was determined to be 8.91 ng/mL in A549 cell extract based on a 2.20 mg/mL extract load, 11.75 ng/mL in HeLa cell extract based on a 3.20 mg/mL extract load, 9.66 ng/mL in MCF-7 cell extract based on a 4.30 mg/mL extract load and 29.51 ng/mL in K-562 cell extract based on a 4.50 mg/mL extract load.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using G3BP2 Recombinant antibody (82080-4-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 82080-4-RR (same clone as 82080-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82080-4-RR (G3BP2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82080-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using G3BP2 Recombinant antibody (82080-4-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 82080-4-RR (same clone as 82080-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82080-4-RR (G3BP2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82080-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using G3BP2 Recombinant antibody (82080-4-RR)

    IF Staining of HeLa using 82080-4-RR (same clone as 82080-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP2 antibody (82080-4-RR, Clone: 230275D6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82080-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using G3BP2 Recombinant antibody (82080-4-RR)

    FC experiment of U2OS using 82080-4-RR (same clone as 82080-4-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human G3BP2 (82080-4-RR, Clone:230275D6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82080-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00090-3

    Cytometric bead array standard curve of MP00090-3

    Cytometric bead array standard curve of MP00090-3, G3BP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82080-4-PBS. Detection antibody: 82080-2-PBS. Standard: Ag9355. Range: 0.625-20 ng/mL.

  • Cytometric bead array standard curve of MP00090-1

    Cytometric bead array standard curve of MP00090-1

    Cytometric bead array standard curve of MP00090-1, G3BP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82080-4-PBS. Detection antibody: 82080-3-PBS. Standard: Ag9355. Range: 0.625-20 ng/mL.

  • Cytometric bead array standard curve of MP00090-2

    Cytometric bead array standard curve of MP00090-2

    Cytometric bead array standard curve of MP00090-2, G3BP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82080-4-PBS. Detection antibody: 82080-5-PBS. Standard: Ag9355. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 82080-4-PBS

    Affinity and Kinetic Characterization of 82080-4-PBS

    Biolayer interferometry (BLl) kinetic assay of 82080-4-PBS against Human G3BP2 was performed. The affinity constant is 3.48 nM.

  • Affinity and Kinetic Characterization of 82080-4-RR

    Affinity and Kinetic Characterization of 82080-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82080-4-RR against Human G3BP2 were performed. The affinity constant is 59.6 nM.

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G3BP2 Recombinant antibody, PBS Only (Capture)

G3BP2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 82080-4-PBS
Clone No.230275D6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

G3BP 2, 230275D6, Ras GTPase-activating protein-binding protein 2, GAP SH3 domain-binding protein 2, GAP SH3 domain binding protein 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82080-4-PBS targets G3BP2 as part of a matched antibody pair:

MP00090-1: 82080-4-PBS capture and 82080-3-PBS detection (validated in Cytometric bead array)

MP00090-2: 82080-4-PBS capture and 82080-5-PBS detection (validated in Cytometric bead array)

MP00090-3: 82080-4-PBS capture and 82080-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen G3BP2 fusion protein Ag9355 相同性解析による交差性が予測される生物種
Full Name GTPase activating protein (SH3 domain) binding protein 2
Calculated molecular weight482aa,54 kDa; 449aa,51 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC011731
Gene Symbol G3BP2
Gene ID (NCBI) 9908
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UN86
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer.