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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

G3BP2 Recombinant antibody

G3BP2 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230275D6

Cat no : 82080-4-RR

Synonyms

G3BP 2, 230275D6, Ras GTPase-activating protein-binding protein 2, GAP SH3 domain-binding protein 2, GAP SH3 domain binding protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using G3BP2 Recombinant antibody (82080-4-RR)

    WB analysis using 82080-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82080-4-RR (G3BP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using G3BP2 Recombinant antibody (82080-4-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 82080-4-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82080-4-RR (G3BP2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using G3BP2 Recombinant antibody (82080-4-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 82080-4-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82080-4-RR (G3BP2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using G3BP2 Recombinant antibody (82080-4-RR)

    IF Staining of HeLa using 82080-4-RR

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP2 antibody (82080-4-RR, Clone: 230275D6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of U2OS cells using G3BP2 Recombinant antibody (82080-4-RR)

    FC experiment of U2OS using 82080-4-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human G3BP2 (82080-4-RR, Clone:230275D6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82080-4-RR

    Affinity and Kinetic Characterization of 82080-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82080-4-RR against Human G3BP2 were performed. The affinity constant is 59.6 nM.

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, A549 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected insodium arsenite treated HeLa cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82080-4-RR targets G3BP2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen G3BP2 fusion protein Ag9355 相同性解析による交差性が予測される生物種
Full Name GTPase activating protein (SH3 domain) binding protein 2
Calculated molecular weight482aa,54 kDa; 449aa,51 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC011731
Gene symbol G3BP2
Gene ID (NCBI) 9908
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer.

Protocols

Product Specific Protocols
WB protocol for G3BP2 antibody 82080-4-RRDownload protocol
IHC protocol for G3BP2 antibody 82080-4-RRDownload protocol
IF protocol for G3BP2 antibody 82080-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols