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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated G3BP2 Polyclonal antibody

G3BP2 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16276

Synonyms

G3BP 2, G3BP2, KIAA0660

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated G3BP2 Polyclonal ant (CL488-16276)

    IF Staining of HeLa using CL488-16276

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CoraLite® Plus 488 G3BP2 antibody (CL488-16276) at dilution of 1:200, CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated G3BP2 Polyclonal ant (CL488-16276)

    IF Staining of HeLa using CL488-16276

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CoraLite® Plus 488 G3BP2 antibody (CL488-16276) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

Positive IF/ICC detected insodium arsenite treated HeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-16276 is G3BP2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen G3BP2 fusion protein Ag9355 相同性解析による交差性が予測される生物種
Full Name GTPase activating protein (SH3 domain) binding protein 2
Calculated molecular weight482aa,54 kDa; 449aa,51 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC011731
Gene symbol G3BP2
Gene ID (NCBI) 9908
RRIDAB_2919132
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 G3BP2 antibody CL488-16276Download protocol
Standard Protocols
Click here to view our Standard Protocols