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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated G6PD Monoclonal antibody

G6PD Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

2A7B12

Cat no : CL555-66373

Synonyms

Glucose-6-phosphate 1-dehydrogenase, G6PD1, G6P, EC:1.1.1.49, 2A7B12

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®555-conjugated G6PD Monoclonal antibody (CL555-66373)

    IF Staining of HeLa using CL555-66373

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®555 G6PD antibody (CL555-66373, Clone: 2A7B12 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®555-conjugated G6PD Monoclonal antibody (CL555-66373)

    FC experiment of HeLa using CL555-66373

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human G6PD (CL555-66373, Clone:2A7B12) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66373 targets G6PD in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen G6PD fusion protein Ag21862 相同性解析による交差性が予測される生物種
Full Name glucose-6-phosphate dehydrogenase
Calculated molecular weight 59 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC000337
Gene symbol G6PD
Gene ID (NCBI) 2539
RRIDAB_2919684
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

G6PD(Glucose-6-phosphate 1-dehydrogenase) belongs to the glucose-6-phosphate dehydrogenase family. It is a housekeeping enzyme encoded in mammals by an X-linked gene. G6PD has important functions in intermediary metabolism because it catalyzes the first step in the pentose phosphate pathway and provides reductive potential in the form of NADPH.(PMID:7489710). This protein has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for CL555 G6PD antibody CL555-66373Download protocol
FC protocol for CL555 G6PD antibody CL555-66373Download protocol
Standard Protocols
Click here to view our Standard Protocols