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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GADD45A Recombinant antibody (84376-4-RR)

    WB analysis using 84376-4-RR (same clone as 84376-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84376-4-RR (GADD45A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84376-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of K562 using GADD45A Recombinant antibody (84376-4-RR)

    FC experiment of K562 using 84376-4-RR (same clone as 84376-4-PBS)

    1x10^6 K562 were intracellularly stained with 0.25 ug GADD45A Recombinant antibody (84376-4-RR, Clone:241597H4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84376-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-2 OS using GADD45A Recombinant antibody (84376-4-RR)

    FC experiment of U-2 OS using 84376-4-RR (same clone as 84376-4-PBS)

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug GADD45A Recombinant antibody (84376-4-RR, Clone:241597H4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84376-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01247-2

    Cytometric bead array standard curve of MP01247-2

    Cytometric bead array standard curve of MP01247-2, GADD45A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84376-4-PBS. Detection antibody: 84376-1-PBS. Standard: Ag4687. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84376-4-RR

    Affinity and Kinetic Characterization of 84376-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84376-4-RR against Human GADD45A were performed. The affinity constant is 0.769 nM.

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GADD45A Recombinant antibody, PBS Only (Capture)

GADD45A Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 84376-4-PBS
Clone No.241597H4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

GADD45, DNA damage-inducible transcript 1 protein, DDIT-1, DDIT1, DDIT 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84376-4-PBS targets GADD45A as part of a matched antibody pair:

MP01247-2: 84376-4-PBS capture and 84376-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GADD45A fusion protein Ag4687 相同性解析による交差性が予測される生物種
Full Name growth arrest and DNA-damage-inducible, alpha
Calculated molecular weight 165 aa, 18 kDa
Observed molecular weight14-18 kDa, 28-36 kDa
GenBank accession numberBC011757
Gene Symbol GADD45A
Gene ID (NCBI) 1647
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP24522
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GADD45A, also named as DDIT1 and GADD45, belongs to the GADD45 family. It binds to proliferating cell nuclear antigen. GADD45A may affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. GADD45A plays an essential role in active DNA demethylation during terminal osteogenic differentiation of adipose-derived mesenchymal stem cells. GADD45A has Dimer (~28-36 kDa) and Monomer (14-18 kDa) forms (PMID:11498536).