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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GADD45A Recombinant antibody

GADD45A Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241597H4

Cat no : 84376-4-RR

Synonyms

GADD45, DNA damage-inducible transcript 1 protein, DDIT-1, DDIT1, DDIT 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GADD45A Recombinant antibody (84376-4-RR)

    WB analysis using 84376-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84376-4-RR (GADD45A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of K562 using GADD45A Recombinant antibody (84376-4-RR)

    FC experiment of K562 using 84376-4-RR

    1x10^6 K562 were intracellularly stained with 0.25 ug GADD45A Recombinant antibody (84376-4-RR, Clone:241597H4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Flow cytometry (FC) experiment of U-2 OS using GADD45A Recombinant antibody (84376-4-RR)

    FC experiment of U-2 OS using 84376-4-RR

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug GADD45A Recombinant antibody (84376-4-RR, Clone:241597H4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84376-4-RR

    Affinity and Kinetic Characterization of 84376-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84376-4-RR against Human GADD45A were performed. The affinity constant is 0.769 nM.

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Tested Applications

Positive WB detected inK-562 cells, NIH/3T3 cells
Positive FC (Intra) detected inU-2 OS, K562

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84376-4-RR is GADD45A Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GADD45A fusion protein Ag4687 相同性解析による交差性が予測される生物種
Full Name growth arrest and DNA-damage-inducible, alpha
Calculated molecular weight 165 aa, 18 kDa
Observed molecular weight14-18 kDa, 28-36 kDa
GenBank accession numberBC011757
Gene symbol GADD45A
Gene ID (NCBI) 1647
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GADD45A, also named as DDIT1 and GADD45, belongs to the GADD45 family. It binds to proliferating cell nuclear antigen. GADD45A may affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. GADD45A plays an essential role in active DNA demethylation during terminal osteogenic differentiation of adipose-derived mesenchymal stem cells. GADD45A has Dimer (~28-36 kDa) and Monomer (14-18 kDa) forms (PMID:11498536).

Protocols

Product Specific Protocols
WB protocol for GADD45A antibody 84376-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols