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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 594-conjugated GALNT2 Polyclonal an (CL594-17441)

    IF Staining of HeLa using CL594-17441

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 594 GALNT2 antibody (CL594-17441) at dilution of 1:200.

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CoraLite® Plus 594-conjugated GALNT2 Polyclonal antibody

GALNT2 Polyclonal Antibody for IF/ICC
Cat No. CL594-17441

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

GalNAc T2, GALNT2, pp GaNTase 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 594
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-17441 targets GALNT2 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GALNT2 fusion protein Ag11523 相同性解析による交差性が予測される生物種
Full Name UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (GalNAc-T2)
Calculated molecular weight 571 aa, 65 kDa
Observed molecular weight64 kDa
GenBank accession numberBC041120
Gene Symbol GALNT2
Gene ID (NCBI) 2590
RRIDAB_3673427
Conjugate CoraLite® Plus 594 Fluorescent Dye
Excitation/Emission maxima wavelengths594 nm / 615 nm
Form Liquid
Purification MethodAntigen Affinity Purified
UNIPROT IDQ10471
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GALNT2, a GalNAc-transferase involved in O-linked glycosylation, modulates the risk of atherogenic dyslipidemia, a specific hallmark of insulin resistance and acts as a positive modulator of insulin signaling in human liver cells by down-regulating ENPP1. GALNT2 gene encodes GalNAc-T2 as a regulator of high-density lipoprotein cholesterol (HDL-C) metabolism (PMID: 31040393, 27508872). Western blot analysis detected GALNT2 at an apparent molecular mass of 64 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 594 GALNT2 antibody CL594-17441Download protocol
Standard Protocols
Click here to view our Standard Protocols