Validation Data Gallery
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Tested Applications
| Positive WB detected in | fetal human brain tissue, mouse liver tissue, pig liver tissue, rat liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
Product Information
66322-1-Ig targets GAMT in WB, ELISA applications and shows reactivity with human, rat, mouse, pig samples.
| Tested Reactivity | human, rat, mouse, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | GAMT fusion protein Ag16778 相同性解析による交差性が予測される生物種 |
| Full Name | guanidinoacetate N-methyltransferase |
| Calculated molecular weight | 26 kDa |
| Observed molecular weight | 26 kDa |
| GenBank accession number | BC016760 |
| Gene Symbol | GAMT |
| Gene ID (NCBI) | 2593 |
| RRID | AB_2881702 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14353 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GAMT converts the latter to creatine with S-adenosylmethionine as the methyl donor. GAMT was induced in human and mouse cells in response to genotoxic and metabolic stress. GAMT was required for p53-dependent upregulation of creatine biosynthesis, fatty acid oxidation, and apoptosis in response to glucose deprivation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GAMT antibody 66322-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Food Funct The protective effect of polyunsaturated fatty acid intake during pregnancy against embryotoxicity of sodium valproate in mice | ||
Sci Rep Intratesticular creatine maintains spermatogenesis by defining tight junctions
| ||
Sci Rep Identification of disulfidptosis-related subtypes in gastric cancer and GAMT is a key gene during disulfidptosis
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