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Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using GAMT Monoclonal antibody (66322-1-Ig)

    WB analysis of fetal human brain using 66322-1-Ig (same clone as 66322-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66322-1-Ig (GAMT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66322-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using GAMT Monoclonal antibody (66322-1-Ig)

    WB analysis of mouse liver using 66322-1-Ig (same clone as 66322-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 66322-1-Ig (GAMT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66322-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using GAMT Monoclonal antibody (66322-1-Ig)

    WB analysis of rat liver using 66322-1-Ig (same clone as 66322-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 66322-1-Ig (GAMT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66322-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using GAMT Monoclonal antibody (66322-1-Ig)

    WB analysis of pig liver using 66322-1-Ig (same clone as 66322-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66322-1-Ig (GAMT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66322-1-PBS in a different storage buffer formulation.

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GAMT Monoclonal antibody, PBS Only

GAMT Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66322-1-PBS
Clone No.1E7B11

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, mouse, pig

Applications

WB, Indirect ELISA

Synonyms

GAMT, PIG2, TP53I2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66322-1-PBS targets GAMT in WB, Indirect ELISA applications and shows reactivity with human, rat, mouse, pig samples.

Tested Reactivity human, rat, mouse, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GAMT fusion protein Ag16778 相同性解析による交差性が予測される生物種
Full Name guanidinoacetate N-methyltransferase
Calculated molecular weight 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC016760
Gene Symbol GAMT
Gene ID (NCBI) 2593
RRIDAB_2881702
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14353
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GAMT converts the latter to creatine with S-adenosylmethionine as the methyl donor. GAMT was induced in human and mouse cells in response to genotoxic and metabolic stress. GAMT was required for p53-dependent upregulation of creatine biosynthesis, fatty acid oxidation, and apoptosis in response to glucose deprivation.