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GAPDH 抗体 5067 文献

Mouse Monoclonal| カタログ番号: 60004-1-Ig |クローン番号: 1E6D9

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Related Primary Antibodies

適用アプリケーション

アプリケーション:
WB, IP, IHC, IF, FC, CoIP, ChIP
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Images See all

GAPDH Antibody WB HeLa cells 60004-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

GAPDH Antibody WB ROS1728 cells 60004-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

GAPDH Antibody WB pig brain tissue 60004-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

GAPDH Antibody WB HepG2 cells 60004-1-Ig

Western blot analysis of GAPDH in various tissues and cell lines using Proteintech antibody 60004-1-Ig at a dilution of 1:10000.

GAPDH Antibody IP HeLa Cells 60004-1-Ig

IP result of anti-GAPDH (60004-1-Ig for IP and Detection) with HeLa cell lysate.

GAPDH Antibody WB arabidopsis whole plant tissue 60004-1-Ig

arabidopsis, rice, wheat, corn whole plant tissue were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

GAPDH Antibody IF Ethacrynic acid treated HeLa cells 60004-1-Ig

Immunofluorescent analysis of EA treated HeLa cells using 60004-1-Ig(GAPDH antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-mouse IgG (red).

GAPDH Antibody FC HeLa cells 60004-1-Ig

1X10^6 HeLa cells were stained with 0.2ug GAPDH antibody (60004-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Mouse IgG with dilution 1:100.

種特異性:
human, mouse, rat, yeast, plant, zebrafish, Arabidopsis, Bombyx mori, bovine, Branchiostoma belcheri, brovine, Caenorhabditis elegans, canine, chicken, CHO cell line, Cynomorium songaricum
WB ポジティブ:
HeLa cells, soybean whole plant tissue, arabidopsis whole plant tissue, HepG2 cells, ROS1728 cells, pig brain tissue, zebrafish tissue, whole yeast cells, whole Nematode tissue, HEK-293 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, C2C12 cells, SP2/0 cells, rat brain tissue, mouse brain tissue
IP ポジティブ:
HeLa Cells
IF ポジティブ:
Ethacrynic acid treated HeLa cells
FC ポジティブ:
HeLa cells
推奨希釈倍率:
WB : 1:20000-1:100000
IP : 0.5-4.0 ug for IP and 1:2000-1:12000 for WB
IF : 1:200-1:2000

Protocols

Product-specific protocols

Standard Protocols

製品情報

由来:
Mouse
精製方法:
Protein A purification
アイソタイプ:
IgG2b
バッファー/保存方法:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

ターゲット情報

免疫原:
GAPDH fusion protein Ag0766
正式名:
glyceraldehyde-3-phosphate dehydrogenase
計算分子量:
36 kDa
検出分子量:
36 kDa
GenBank accession number:
BC004109
Gene ID (NCBI):
2597
遺伝子シンボル:
GAPDH
別名:
G3PD, GAPD, GAPDH, OK/SW cl.12

背景説明

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable.


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