GAPDH Monoclonal antibody

A nice antibody to detect GAPDH in both mouse cortex lysates.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse cortex lysate

Not applicable

• Applications: Western Blot
• Cell Tissue Type: HeLa cell

Great antibody! Clear band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000-10000
• Cell Tissue Type: mouse BMDM

Membrane was blocked for 1 hour with 5% nonfat milk in TBS + 0.01% Tween, then probed overnight at 4C with a 1:20,000 dilution of a-GAPDH antibody in blocking buffer. After washing, the membrane was probed with an a-mouse secondary antibody at 1:50,000 in TBST. Under these conditions, the signal is very robust, even at a short exposure (~30 seconds) and has low background. The GAPDH antibody could likely be used at lower concentrations. Quite pleased with how it looks!

• Applications: Western Blot
• Primary Antibody Dilution: 1:20000
• Cell Tissue Type: Xenopus Embryo Extract

Excellent antibody! It gives clean and specific results

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T

This GAPDH Monoclonal antibody Cat No. 60004-1-Ig works OK.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293

This antibody is very good. We have used it many times and with really good results.

• Applications: Western Blot, Cell culture
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Ovarian cancer stem cells,

Good antibody for WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: Mouse Heart

293T lysate (20 ug) was loaded on 4-12% Bis-Tris gels and transferred to PVDF membrane. Membrane was incubated in 5% BSA in PBS/0.1% Tween-20 for 1h. Membrane was incubated with anti-GAPDH in blocking buffer (1:5000) at 4 degree overnight. After washing, membrane was incubated in anti-mouse-HRP in blocking buffer (1:10000) for 1h at room temperature. Membrane was developed in ECL and imaged on a Biorad chemiluminescence detection system.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: 293T

This antibody worked quite well in my western blotting. I used 5% skim milk TBS-T for blocking and antibody dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: hTERT-RPE1

HEK293T lysate (10ug) ran on 4-20% gradient gel. Blocking in 5% BSA for 1 hour, followed by HSP27 (1:5000 in block) overnight at 4 degrees. Goat anti-mouse HRP (1:10,000) for 1 hour in block.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293T

Excellent antibody.I used in WB on HeLa lysate and the results was extremely clear. No unspecific bands detected. Perfect as loading control.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: HeLa cells

Good antibody for Western BLOT. antibody diluted in 5%milk.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK 293T

Good signal for WB

• Applications: Western Blot,
• Cell Tissue Type: HEKs

Excellent antibody. Single band at the correct molecular weight. No background and can be used at high dilutions.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: SH-SY5Y

Need a 1 hr block with 5% milk / TBST as usual.1 hr room temp with the same blocking media with a 1:8000 dilution, followed by secondary anti mouse ( same dilution) , HRP conjugated 1 hr room temp.Detect and document.4 is rating , since a 1:8000 fold is tad bit low, have used better dilution ( 1:100,000) in the past, however that product is not available these days

• Applications: Western Blot,
• Primary Antibody Dilution: 1 to 8000
• Cell Tissue Type: MDA-MB-231

This antibody works well detecting GAPDH in 3t3-L1 and primary mouse embryonic fibroblasts.

• Applications: Western Blot,
• Cell Tissue Type: 3t3-L1, pMEF

High afffinity and specific antibody. Useful for protein controls in western blots.

• Applications: Western Blot,
• Cell Tissue Type: Cardiomyocytes

This antibody works really well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: CHO-K1 cell

WB, it works well in 1:10000 dilution.

• Applications: Western Blot,

Working excellent

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Pancreatic and Colon cancer cells

Excellent antibody. Clean and specific

• Applications: Western Blot,
• Primary Antibody Dilution: 1:25000
• Cell Tissue Type: Protein from mouse tail tendon

Excellent and clean Western blots.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HEK293T

The antibody works good in mouse brain tissue in western blotting.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

Good

• Applications: Western Blot,
• Cell Tissue Type: Ac16

Very good

• Applications: Western Blot,
• Cell Tissue Type: AC16

Total cell lysate (5-20 ug) was resolved on 4-12% Bis-Tris gels and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% BSA in PBS/0.1% Tween-20) for 1h. Membrane was incubated with anti-GAPDH in blocking buffer (1:5000) at 4C overnight. After washing, membrane was incubated in anti-mouse-HRP in blocking bufffer (1:10000) for 2h at room temperature. Membrane was developed in ECF reagent and imaged on a chemiluminescence detection system.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: brain

Excellent antibody! It gives clean and specific results

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: brain lysate

This antibody offers a good detection of GAPDH on mouse cells such as macrophages and microglia through western blots.

• Applications: Western Blot,

Total cell lysate (15 ug) was resolved on 4-12% Bis-Tris gels and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% milk in PBS/0.1% Tween-20) for 1h. Membrane was incubated with anti-GAPDH in blocking buffer (1:10,000) at 4C overnight. After washing, membrane was incubated in anti-mouse-HRP in blocking bufffer (1:5000) for 2h at room temperature. Membrane was developed in ECL reagent and imaged on a chemiluminescence detection system.

• Applications: Western Blot,
• Primary Antibody Dilution: 10,000
• Cell Tissue Type: HeLa cells lysate

great antibody for WB as a loading control used 1:20000

• Applications: Western Blot,
• Primary Antibody Dilution: 1:20000
• Cell Tissue Type: HeLa

I working good for Western Blotting (1:5000)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: H69, HUCCT-1

In muscle cells, GAPDH antibody shows two bands while in cancer cells there is only one band

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C2C12

• Applications: Western Blot,
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: neuronal cell culture

This antibody is useful as a cytoplasmic control for cyto-nuclear extractions.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Prostate cancer cells

• Applications: Western Blot,
• Primary Antibody Dilution: 1:40000
• Cell Tissue Type: Human cell line lysates

The bands of the GADPH looked very good! The antibody used in trophoblast cell and diluted in 1:1000.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Trophoblast

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: colon

very efficient product and great customer service

• Applications: Cell culture,

great sensitivity and reproducibility

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: macrophage

• Applications: Western Blot,
• Primary Antibody Dilution: 1:8000
• Cell Tissue Type: Human breast cancer

• Applications: Western Blot,
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: Huh7 cells

Worked well in the western blot

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human THP1