- Featured Product
- KD/KO Validated
GAPDH Monoclonal antibody, PBS Only
GAPDH Monoclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, pig, zebrafish, yeast, plant
Applications
WB, IF/ICC, FC (Intra), IP, ELISA
Conjugate
Unconjugated
CloneNo.
1E6D9
Cat no : 60004-1-PBS
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
Application | Dilution |
---|---|
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
Product Information
60004-1-PBS targets GAPDH in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, pig, zebrafish, yeast, plant samples.
Tested Reactivity | human, mouse, rat, pig, zebrafish, yeast, plant |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | GAPDH fusion protein Ag0766 相同性解析による交差性が予測される生物種 |
Full Name | glyceraldehyde-3-phosphate dehydrogenase |
Calculated molecular weight | 36 kDa |
Observed molecular weight | 36 kDa |
GenBank accession number | BC004109 |
Gene symbol | GAPDH |
Gene ID (NCBI) | 2597 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. |
Background Information
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable.