CoraLite® Plus 647-conjugated GAPDH (Human Specific) Recombinant antibody
GAPDH (Human Specific) Recombinant Antibody for WB, IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
2G21
Cat no : CL647-80570
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cells |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL647-80570 targets GAPDH (Human Specific) in WB, IF/ICC, FC (Intra) applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | GAPDH (Human Specific) fusion protein Ag0766 相同性解析による交差性が予測される生物種 |
Full Name | glyceraldehyde-3-phosphate dehydrogenase |
Calculated molecular weight | 36 kDa |
Observed molecular weight | 33 kDa |
GenBank accession number | BC004109 |
Gene symbol | GAPDH |
Gene ID (NCBI) | 2597 |
RRID | AB_2920334 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable. 80570-1-RR is human specific.
Protocols
Product Specific Protocols | |
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WB protocol for CL Plus 647 GAPDH (Human Specific) antibody CL647-80570 | Download protocol |
IF protocol for CL Plus 647 GAPDH (Human Specific) antibody CL647-80570 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |