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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GAS2L1 Monoclonal antibody

GAS2L1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C6D5

Cat no : 68051-1-Ig

Synonyms

GAR22, GAS2 like protein 1, GAS2L1, growth arrest 2 like 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GAS2L1 Monoclonal antibody (68051-1-Ig)

    WB analysis using 68051-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68051-1-Ig (GAS2L1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of mouse testis tissue using GAS2L1 Monoclonal antibody (68051-1-Ig)

    WB analysis of mouse testis using 68051-1-Ig

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 68051-1-Ig (GAS2L1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using GAS2L1 Monoclonal antibody (68051-1-Ig)

    IHC staining of mouse testis using 68051-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68051-1-Ig (GAS2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using GAS2L1 Monoclonal antibody (68051-1-Ig)

    IHC staining of mouse testis using 68051-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68051-1-Ig (GAS2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells, mouse testis tissue, K-562 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68051-1-Ig targets GAS2L1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GAS2L1 fusion protein Ag31232 相同性解析による交差性が予測される生物種
Full Name growth arrest-specific 2 like 1
Calculated molecular weight 73 kDa
Observed molecular weight73 kDa
GenBank accession numberBC011047
Gene symbol GAS2L1
Gene ID (NCBI) 10634
RRIDAB_2918793
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GAS2L1(growth arrest‐specific 2‐like 1) is a component of the cytoskeleton that mediates the interaction between microtubules and microfilament (PMID: 34555082). GAS2L1 contains an N‐terminal actin‐binding calponin homology (CH) domain, a MT lattice‐binding Gas2‐related (GAR) domain and a C‐terminal SxIP motif, which mediates the interaction with MT plus ends via EB proteins. GAS2L1 regulates microtubule dynamics and stability by interacting with microtubule plus-end tracking proteins, such as MAPRE1, to regulate microtubule growth along actin stress fibers (PubMed:24706950).

Protocols

Product Specific Protocols
WB protocol for GAS2L1 antibody 68051-1-IgDownload protocol
IHC protocol for GAS2L1 antibody 68051-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols