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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GAS2L1 Monoclonal antibody (68051-1-Ig)

    WB analysis using 68051-1-Ig (same clone as 68051-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68051-1-Ig (GAS2L1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68051-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using GAS2L1 Monoclonal antibody (68051-1-Ig)

    WB analysis of mouse testis using 68051-1-Ig (same clone as 68051-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 68051-1-Ig (GAS2L1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68051-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using GAS2L1 Monoclonal antibody (68051-1-Ig)

    IHC staining of mouse testis using 68051-1-Ig (same clone as 68051-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68051-1-Ig (GAS2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68051-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using GAS2L1 Monoclonal antibody (68051-1-Ig)

    IHC staining of mouse testis using 68051-1-Ig (same clone as 68051-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68051-1-Ig (GAS2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68051-1-PBS in a different storage buffer formulation.

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GAS2L1 Monoclonal antibody, PBS Only

GAS2L1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 68051-1-PBS
Clone No.2C6D5

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

GAR22, GAS2 like protein 1, GAS2L1, growth arrest 2 like 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68051-1-PBS targets GAS2L1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GAS2L1 fusion protein Ag31232 相同性解析による交差性が予測される生物種
Full Name growth arrest-specific 2 like 1
Calculated molecular weight 73 kDa
Observed molecular weight73 kDa
GenBank accession numberBC011047
Gene Symbol GAS2L1
Gene ID (NCBI) 10634
RRIDAB_2918793
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99501
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GAS2L1(growth arrest‐specific 2‐like 1) is a component of the cytoskeleton that mediates the interaction between microtubules and microfilament (PMID: 34555082). GAS2L1 contains an N‐terminal actin‐binding calponin homology (CH) domain, a MT lattice‐binding Gas2‐related (GAR) domain and a C‐terminal SxIP motif, which mediates the interaction with MT plus ends via EB proteins. GAS2L1 regulates microtubule dynamics and stability by interacting with microtubule plus-end tracking proteins, such as MAPRE1, to regulate microtubule growth along actin stress fibers (PubMed:24706950).