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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using GATA3 Monoclonal antibody (66400-1-Ig)

    WB analysis of MCF-7 using 66400-1-Ig (same clone as 66400-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66400-1-Ig (GATA3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using GATA3 Monoclonal antibody (66400-1-Ig)

    WB analysis of HeLa using 66400-1-Ig (same clone as 66400-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66400-1-Ig (GATA3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using GATA3 Monoclonal antibody (66400-1-Ig)

    WB analysis of RAW 264.7 using 66400-1-Ig (same clone as 66400-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66400-1-Ig (GATA3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using GATA3 Monoclonal antibody (66400-1-Ig)

    WB analysis of Jurkat using 66400-1-Ig (same clone as 66400-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66400-1-Ig (GATA3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GATA3 Monoclonal antibody (66400-1-Ig)

    IHC staining of human breast cancer using 66400-1-Ig (same clone as 66400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66400-1-Ig (GATA3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GATA3 Monoclonal antibody (66400-1-Ig)

    IHC staining of human colon cancer using 66400-1-Ig (same clone as 66400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66400-1-Ig (GATA3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GATA3 Monoclonal antibody (66400-1-Ig)

    IHC staining of human breast cancer using 66400-1-Ig (same clone as 66400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66400-1-Ig (GATA3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GATA3 Monoclonal antibody (66400-1-Ig)

    IHC staining of human colon cancer using 66400-1-Ig (same clone as 66400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66400-1-Ig (GATA3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using GATA3 Monoclonal antibody (66400-1-Ig)

    IF Staining of human breast cancer using 66400-1-Ig (same clone as 66400-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66400-1-Ig (GATA3 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using GATA3 Monoclonal antibody (66400-1-Ig)

    IF Staining of human breast cancer using 66400-1-Ig (same clone as 66400-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66400-1-Ig (GATA3 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using GATA3 Monoclonal antibody (66400-1-Ig)

    IF Staining of MCF-7 using 66400-1-Ig (same clone as 66400-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GATA3 antibody (66400-1-Ig, Clone: 1B11A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GATA3 Monoclonal antibody (66400-1-Ig)

    IF Staining of HepG2 using 66400-1-Ig (same clone as 66400-1-PBS)

    Immunofluorescent analysis of (4% PFA ) fixed HepG2 cells using 66400-1-Ig(GATA3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

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GATA3 Monoclonal antibody, PBS Only

GATA3 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 66400-1-PBS
Clone No.1B11A8

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 1B11A8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66400-1-PBS targets GATA3 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GATA3 fusion protein Ag0174 相同性解析による交差性が予測される生物種
Full Name GATA binding protein 3
Calculated molecular weight 443 aa, 48 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC003070
Gene Symbol GATA3
Gene ID (NCBI) 2625
RRIDAB_2881774
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23771
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GATA3 is a transcriptional factor that binds to the enhancer elements of all 4 T-cell antigen receptor genes. GATA3 is highly expressed in naive, freshly activated cells and Th2 lineage cells and thought to be necessary and sufficient for Th2 cytokine gene expression. GATA3 and TBET interfer with each other to regulate gene expression.