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GATA3 Monoclonal antibody

GATA3 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 66400-1-Ig
Clone No.1B11A8

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, IF-P, CoIP, ELISA

Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 1B11A8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, Jurkat cells, RAW 264.7 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman breast cancer tissue
Positive IF/ICC detected inMCF-7 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66400-1-Ig targets GATA3 in WB, IHC, IF/ICC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GATA3 fusion protein Ag0174 相同性解析による交差性が予測される生物種
Full Name GATA binding protein 3
Calculated molecular weight 443 aa, 48 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC003070
Gene Symbol GATA3
Gene ID (NCBI) 2625
RRIDAB_2881774
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23771
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GATA3 is a transcriptional factor that binds to the enhancer elements of all 4 T-cell antigen receptor genes. GATA3 is highly expressed in naive, freshly activated cells and Th2 lineage cells and thought to be necessary and sufficient for Th2 cytokine gene expression. GATA3 and TBET interfer with each other to regulate gene expression.

Protocols

Product Specific Protocols
WB protocol for GATA3 antibody 66400-1-IgDownload protocol
IHC protocol for GATA3 antibody 66400-1-IgDownload protocol
IF protocol for GATA3 antibody 66400-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Drug Resist Updat

GATA3 mediates doxorubicin resistance by inhibiting CYB5R2-catalyzed iron reduction in breast cancer cells

Authors - Zhen Zhu
humanWB

Cardiovasc Diabetol

BAG3 promotes proliferation and migration of arterial smooth muscle cells by regulating STAT3 phosphorylation in diabetic vascular remodeling

Authors - Xinyue Huang
pigWB

J Ethnopharmacol

Therapeutic effects and mechanisms study of Hanchuan Zupa Granule in a Guinea pig model of cough variant asthma.

Authors - Pengfei Li
mouseWB

Pharm Biol

Modified BuShenYiQi formula alleviates experimental allergic asthma in mice by negative regulation of type 2 innate lymphoid cells and CD4 + type 9 helper T cells and the VIP-VPAC2 signalling pathway

Authors - Muhua Huang
mouseWB

Int Arch Allergy Immunol

Lack of WDFY4 Aggravates Ovalbumin-Induced Asthma via Enhanced Th2 Cell Differentiation.

Authors - Yan Li
mouseWB,IF

Evid Based Complement Alternat Med

Astragali Radix-Coptis Rhizoma Herb Pair Attenuates Atherosclerosis in ApoE-/- Mice by Regulating the M1/M2 and Th1/Th2 Immune Balance and Activating the STAT6 Signaling Pathway.

Authors - Zhaoyu Li
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