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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated GATA3 Monoclonal antibody

GATA3 Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1B11A8

Cat no : CL594-66400

Synonyms

Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 1B11A8

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CoraLite®594-conjugated GATA3 Monoclonal antibody (CL594-66400)

    IF Staining of human breast cancer using CL594-66400

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CL594-66400 (GATA3 antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CoraLite®594-conjugated GATA3 Monoclonal antibody (CL594-66400)

    IF Staining of human breast cancer using CL594-66400

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CL594-66400 (GATA3 antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite®594-conjugated GATA3 Monoclonal antibody (CL594-66400)

    IF Staining of MCF-7 using CL594-66400

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 GATA3 antibody (CL594-66400, Clone: 1B11A8 ) at dilution of 1:800, CL488-Phalloidin (green).

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Tested Applications

Positive IF-P detected inhuman breast cancer tissue
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66400 targets GATA3 in IF/ICC, IF-P applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GATA3 fusion protein Ag0174 相同性解析による交差性が予測される生物種
Full Name GATA binding protein 3
Calculated molecular weight 443 aa, 48 kDa
GenBank accession numberBC003070
Gene symbol GATA3
Gene ID (NCBI) 2625
RRIDAB_2883576
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GATA3 is a transcriptional factor that binds to the enhancer elements of all 4 T-cell antigen receptor genes. GATA3 is highly expressed in naive, freshly activated cells and Th2 lineage cells and thought to be necessary and sufficient for Th2 cytokine gene expression. GATA3 and TBET interfer with each other to regulate gene expression. This antibody is CL594(Ex/Em 593 nm/614 nm) conjugated.

Protocols

Product Specific Protocols
IF protocol for CL594 GATA3 antibody CL594-66400Download protocol
Standard Protocols
Click here to view our Standard Protocols