Validation Data Gallery
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Tested Applications
| Positive WB detected in | pig kidney tissue, rat kidney tissue, mouse kidney tissue, pig pancreas tissue, rat pancreas tissue, mouse pancreas tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68513-1-Ig targets GATM in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | GATM fusion protein Ag3754 相同性解析による交差性が予測される生物種 |
| Full Name | glycine amidinotransferase (L-arginine:glycine amidinotransferase) |
| Calculated molecular weight | 423 aa, 48 kDa |
| Observed molecular weight | 48 kDa |
| GenBank accession number | BC004141 |
| Gene Symbol | GATM |
| Gene ID (NCBI) | 2628 |
| RRID | AB_3085222 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P50440 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GATM, also known as AGAT, produces ornithine and guanidinoacetate (GAA) from arginine and glycine, and is a key enzyme for creatine synthesis. GATM deficiency in early infancy causes neurodevelopmental delay. GATM is predominantly expressed in kidney and pancreas in adults. Decrease of GAMT proteins was observed in proximal tubule damage cases, which links GATM as a general marker for proximal tubule damage. Alternative splicing of GATM generates three isoforms with molecular weights between 44 kDa to 48 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GATM antibody 68513-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |