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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GATM Monoclonal antibody, PBS Only

GATM Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D12G5

Cat no : 68513-1-PBS

Synonyms

Transamidinase, L-arginine:glycine amidinotransferase, EC:2.1.4.1, AGAT, 1D12G5

Validation Data Gallery

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  • Western Blot (WB) analysis of pig kidney tissue using GATM Monoclonal antibody (68513-1-Ig)

    WB analysis of pig kidney using 68513-1-Ig (same clone as 68513-1-PBS)

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 68513-1-Ig (GATM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68513-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GATM Monoclonal antibody (68513-1-Ig)

    WB analysis using 68513-1-Ig (same clone as 68513-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68513-1-Ig (GATM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68513-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68513-1-PBS targets GATM in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GATM fusion protein Ag3754 相同性解析による交差性が予測される生物種
Full Name glycine amidinotransferase (L-arginine:glycine amidinotransferase)
Calculated molecular weight 423 aa, 48 kDa
Observed molecular weight48 kDa
GenBank accession numberBC004141
Gene symbol GATM
Gene ID (NCBI) 2628
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GATM, also known as AGAT, produces ornithine and guanidinoacetate (GAA) from arginine and glycine, and is a key enzyme for creatine synthesis. GATM deficiency in early infancy causes neurodevelopmental delay. GATM is predominantly expressed in kidney and pancreas in adults. Decrease of GAMT proteins was observed in proximal tubule damage cases, which links GATM as a general marker for proximal tubule damage. Alternative splicing of GATM generates three isoforms with molecular weights between 44 kDa to 48 kDa.