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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GBP3 Recombinant antibody (83534-1-RR)

    WB analysis using 83534-1-RR (same clone as 83534-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83534-1-RR (GBP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83534-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HT-29 cells using GBP3 Recombinant antibody (83534-1-RR)

    FC experiment of HT-29 using 83534-1-RR (same clone as 83534-1-PBS)

    1x10^6 HT-29 cells were intracellularly stained with 0.25 ug Gbp3 Recombinant Antibody (83534-1-RR, Clone:230178D8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83534-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83534-1-RR

    Affinity and Kinetic Characterization of 83534-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83534-1-RR against Human GBP3 were performed. The affinity constant is below 1 pM.

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GBP3 Recombinant antibody, PBS Only

GBP3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83534-1-PBS
Clone No.230178D8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

GTP-binding protein 3, GTP binding protein 3, GBP-3, GBP 3, EC:3.6.5.-

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83534-1-PBS targets GBP3 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GBP3 fusion protein Ag33889 相同性解析による交差性が予測される生物種
Full Name guanylate binding protein 3
GenBank accession numberBC140837
Gene Symbol GBP3
Gene ID (NCBI) 2635
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9H0R5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.