Validation Data Gallery
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Tested Applications
| Positive WB detected in | K-562 cells, HeLa cells, Jurkat cells, HUVEC cells, U-251 cells |
| Positive FC (Intra) detected in | HT-29 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83534-1-RR targets GBP3 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | GBP3 fusion protein Ag33889 相同性解析による交差性が予測される生物種 |
| Full Name | guanylate binding protein 3 |
| Observed molecular weight | 62 kDa, 68 kDa |
| GenBank accession number | BC140837 |
| Gene Symbol | GBP3 |
| Gene ID (NCBI) | 2635 |
| RRID | AB_3671159 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q9H0R5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Guanylate-binding protein 3 (GBP3) is involved in the proliferation of glioma cells through regulating SQSTM1-ERK1/2 pathway. GBP3 has 2 isoforms, one is 595 amino acids long at 68 kDa and the other is at nearly 62 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GBP3 antibody 83534-1-RR | Download protocol |
| FC protocol for GBP3 antibody 83534-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |