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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GBP3 Recombinant antibody

GBP3 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230178D8

Cat no : 83534-1-RR

Synonyms

GTP-binding protein 3, GTP binding protein 3, GBP-3, GBP 3, EC:3.6.5.-

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GBP3 Recombinant antibody (83534-1-RR)

    WB analysis using 83534-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83534-1-RR (GBP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HT-29 cells using GBP3 Recombinant antibody (83534-1-RR)

    FC experiment of HT-29 using 83534-1-RR

    1x10^6 HT-29 cells were intracellularly stained with 0.25 ug Gbp3 Recombinant Antibody (83534-1-RR, Clone:230178D8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83534-1-RR

    Affinity and Kinetic Characterization of 83534-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83534-1-RR against Human GBP3 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inK-562 cells, HeLa cells, Jurkat cells, HUVEC cells, U-251 cells
Positive FC (Intra) detected inHT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83534-1-RR is GBP3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GBP3 fusion protein Ag33889 相同性解析による交差性が予測される生物種
Full Name guanylate binding protein 3
Observed molecular weight62 kDa, 68 kDa
GenBank accession numberBC140837
Gene symbol GBP3
Gene ID (NCBI) 2635
RRIDAB_3671159
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Guanylate-binding protein 3 (GBP3) is involved in the proliferation of glioma cells through regulating SQSTM1-ERK1/2 pathway. GBP3 has 2 isoforms, one is 595 amino acids long at 68 kDa and the other is at nearly 62 kDa.

Protocols

Product Specific Protocols
WB protocol for GBP3 antibody 83534-1-RRDownload protocol
FC protocol for GBP3 antibody 83534-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols