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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GBP4 Recombinant antibody (83699-4-RR)

    WB analysis of HeLa using 83699-4-RR (same clone as 83699-4-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 83699-4-RR (GBP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83699-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using GBP4 Recombinant antibody (83699-4-RR)

    FC experiment of HepG2 using 83699-4-RR (same clone as 83699-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug GBP4 Recombinant antibody (83699-4-RR, Clone:240730H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83699-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83699-4-RR

    Affinity and Kinetic Characterization of 83699-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83699-4-RR against Human GBP4 were performed. The affinity constant is 11.6 pM.

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GBP4 Recombinant antibody, PBS Only

GBP4 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83699-4-PBS
Clone No.240730H5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

Guanylate-binding protein 4, Guanine nucleotide-binding protein 4, GTP-binding protein 4, GBP-4, EC:3.6.5.-

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83699-4-PBS targets GBP4 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GBP4 fusion protein Ag12006 相同性解析による交差性が予測される生物種
Full Name guanylate binding protein 4
Calculated molecular weight 640 aa, 73 kDa
Observed molecular weight 73 kDa
GenBank accession numberBC070055
Gene Symbol GBP4
Gene ID (NCBI) 115361
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96PP9
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Guanylate-binding protein 4 (GBP4) is an IFN-inducible GTPase that belongs to the GB1/RHD3 GTPase family. GBP4 plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens. GBP4 negatively regulates the antiviral response by inhibiting activation of IRF7 transcription factor. The molecular weight of GBP4 is 73 kDa.