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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GBP4 Recombinant antibody (83699-4-RR)

    WB analysis of HeLa using 83699-4-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 83699-4-RR (GBP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HepG2 cells using GBP4 Recombinant antibody (83699-4-RR)

    FC experiment of HepG2 using 83699-4-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug GBP4 Recombinant antibody (83699-4-RR, Clone:240730H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83699-4-RR

    Affinity and Kinetic Characterization of 83699-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83699-4-RR against Human GBP4 were performed. The affinity constant is 11.6 pM.

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GBP4 Recombinant antibody

GBP4 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83699-4-RR
Clone No.240730H5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

Guanylate-binding protein 4, Guanine nucleotide-binding protein 4, GTP-binding protein 4, GBP-4, EC:3.6.5.-

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83699-4-RR targets GBP4 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GBP4 fusion protein Ag12006 相同性解析による交差性が予測される生物種
Full Name guanylate binding protein 4
Calculated molecular weight 640 aa, 73 kDa
Observed molecular weight 73 kDa
GenBank accession numberBC070055
Gene Symbol GBP4
Gene ID (NCBI) 115361
RRIDAB_3671303
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96PP9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Guanylate-binding protein 4 (GBP4) is an IFN-inducible GTPase that belongs to the GB1/RHD3 GTPase family. GBP4 plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens. GBP4 negatively regulates the antiviral response by inhibiting activation of IRF7 transcription factor. The molecular weight of GBP4 is 73 kDa.

Protocols

Product Specific Protocols
WB protocol for GBP4 antibody 83699-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols