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  • Featured Product
  • KD/KO Validated

GCH1 Polyclonal antibody, PBS Only

GCH1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28501-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GCH1 Polyclonal antibody (28501-1-AP)

    WB analysis using 28501-1-AP (same clone as 28501-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28501-1-AP (GCH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28501-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using GCH1 Polyclonal antibody (28501-1-AP)

    IHC staining of rat liver using 28501-1-AP (same clone as 28501-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 28501-1-AP (GCH1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28501-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using GCH1 Polyclonal antibody (28501-1-AP)

    IHC staining of mouse liver using 28501-1-AP (same clone as 28501-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28501-1-AP (GCH1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28501-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using GCH1 Polyclonal antibody (28501-1-AP)

    IHC staining of mouse liver using 28501-1-AP (same clone as 28501-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28501-1-AP (GCH1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28501-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using GCH1 Polyclonal antibody (28501-1-AP)

    IHC staining of rat liver using 28501-1-AP (same clone as 28501-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 28501-1-AP (GCH1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28501-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28501-1-PBS targets GCH1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GCH1 fusion protein Ag28896 相同性解析による交差性が予測される生物種
Full Name GTP cyclohydrolase 1
Calculated molecular weight 28 kDa
Observed molecular weight 30-35 kDa
GenBank accession numberBC025415
Gene symbol GCH1
Gene ID (NCBI) 2643
RRIDAB_2881157
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.