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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GCK Monoclonal antibody

GCK Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1C3A3

Cat no : 67216-1-Ig

Synonyms

HK IV, Hexokinase-D, Hexokinase-4, Glucokinase, EC:2.7.1.1

Validation Data Gallery

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  • Western Blot (WB) analysis of pig liver tissue using GCK Monoclonal antibody (67216-1-Ig)

    WB analysis of pig liver using 67216-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GCK Monoclonal antibody (67216-1-Ig)

    WB analysis using 67216-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using GCK Monoclonal antibody (67216-1-Ig)

    WB analysis of HepG2 using 67216-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using GCK Monoclonal antibody (67216-1-Ig)

    WB analysis of SMMC-7721 using 67216-1-Ig

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using GCK Monoclonal antibody (67216-1-Ig)

    WB analysis of HSC-T6 using 67216-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using GCK Monoclonal antibody (67216-1-Ig)

    WB analysis of L02 using 67216-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using GCK Monoclonal antibody (67216-1-Ig)

    WB analysis of rat liver using 67216-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GCK Monoclonal antibody (67216-1-Ig)

    WB analysis using 67216-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GCK Monoclonal antibody (67216-1-Ig)

    IHC staining of human liver cancer using 67216-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67216-1-Ig (GCK antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GCK Monoclonal antibody (67216-1-Ig)

    IHC staining of human liver cancer using 67216-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67216-1-Ig (GCK antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using GCK Monoclonal antibody (67216-1-Ig)

    IF Staining of human liver cancer using 67216-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GCK antibody (67216-1-Ig, Clone: 1C3A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using GCK Monoclonal antibody (67216-1-Ig)

    IF Staining of human liver cancer using 67216-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GCK antibody (67216-1-Ig, Clone: 1C3A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inpig liver tissue, SMMC-7721 cells, rat liver tissue, HSC-T6 cells, L02 cells, HepG2 cells, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:300-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67216-1-Ig targets GCK in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GCK fusion protein Ag8116 相同性解析による交差性が予測される生物種
Full Name glucokinase (hexokinase 4)
Calculated molecular weight 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC001890
Gene symbol GCK
Gene ID (NCBI) 2645
RRIDAB_2882508
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glucokinase (GCK) is a structurally and functionally unique member of hexokinase family. It is expressed only in mammalian liver and pancreatic islet beta cells. Because of its unique functional characteristics, the enzyme plays an important regulatory role in glucose metabolism.The rate of glucose metabolism in liver and pancreas is a function of the activity of the enzyme(PMID:1740341). Moreover, GCK has been found to have relationship with diabetes. Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) and familial hyperinsulinemic hypoglycemia type 3 (HHF3). It has 3 isoforms produced by alternative splicing with the same molecular mass of 52 kDa.

Protocols

Product Specific Protocols
WB protocol for GCK antibody 67216-1-IgDownload protocol
IHC protocol for GCK antibody 67216-1-IgDownload protocol
IF protocol for GCK antibody 67216-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Front Pharmacol

Berberrubine, a Main Metabolite of Berberine, Alleviates Non-Alcoholic Fatty Liver Disease via Modulating Glucose and Lipid Metabolism and Restoring Gut Microbiota

Authors - Sa Yang
View Article