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Validation Data Gallery

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  • Western Blot (WB) analysis of pig liver tissue using GCK Monoclonal antibody (67216-1-Ig)

    WB analysis of pig liver using 67216-1-Ig (same clone as 67216-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GCK Monoclonal antibody (67216-1-Ig)

    WB analysis using 67216-1-Ig (same clone as 67216-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using GCK Monoclonal antibody (67216-1-Ig)

    WB analysis of HSC-T6 using 67216-1-Ig (same clone as 67216-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using GCK Monoclonal antibody (67216-1-Ig)

    WB analysis of L02 using 67216-1-Ig (same clone as 67216-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using GCK Monoclonal antibody (67216-1-Ig)

    WB analysis of HepG2 using 67216-1-Ig (same clone as 67216-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SMMC-7721 cells using GCK Monoclonal antibody (67216-1-Ig)

    WB analysis of SMMC-7721 using 67216-1-Ig (same clone as 67216-1-PBS)

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GCK Monoclonal antibody (67216-1-Ig)

    WB analysis using 67216-1-Ig (same clone as 67216-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using GCK Monoclonal antibody (67216-1-Ig)

    WB analysis of rat liver using 67216-1-Ig (same clone as 67216-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GCK Monoclonal antibody (67216-1-Ig)

    IHC staining of human liver cancer using 67216-1-Ig (same clone as 67216-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67216-1-Ig (GCK antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GCK Monoclonal antibody (67216-1-Ig)

    IHC staining of human liver cancer using 67216-1-Ig (same clone as 67216-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67216-1-Ig (GCK antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using GCK Monoclonal antibody (67216-1-Ig)

    IF Staining of human liver cancer using 67216-1-Ig (same clone as 67216-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GCK antibody (67216-1-Ig, Clone: 1C3A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using GCK Monoclonal antibody (67216-1-Ig)

    IF Staining of human liver cancer using 67216-1-Ig (same clone as 67216-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GCK antibody (67216-1-Ig, Clone: 1C3A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

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GCK Monoclonal antibody, PBS Only

GCK Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67216-1-PBS
Clone No.1C3A3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

HK IV, Hexokinase-D, Hexokinase-4, Glucokinase, EC:2.7.1.1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67216-1-PBS targets GCK in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GCK fusion protein Ag8116 相同性解析による交差性が予測される生物種
Full Name glucokinase (hexokinase 4)
Calculated molecular weight 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC001890
Gene Symbol GCK
Gene ID (NCBI) 2645
RRIDAB_2882508
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP35557
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Glucokinase (GCK) is a structurally and functionally unique member of hexokinase family. It is expressed only in mammalian liver and pancreatic islet beta cells. Because of its unique functional characteristics, the enzyme plays an important regulatory role in glucose metabolism.The rate of glucose metabolism in liver and pancreas is a function of the activity of the enzyme(PMID:1740341). Moreover, GCK has been found to have relationship with diabetes. Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) and familial hyperinsulinemic hypoglycemia type 3 (HHF3). It has 3 isoforms produced by alternative splicing with the same molecular mass of 52 kDa.