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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GCS1 Polyclonal antibody (17859-1-AP)

    WB analysis using 17859-1-AP (same clone as 17859-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 17859-1-AP (GCS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17859-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using GCS1 Polyclonal antibody (17859-1-AP)

    WB analysis of COLO 320 using 17859-1-AP (same clone as 17859-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 17859-1-AP (GCS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17859-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using GCS1 Polyclonal antibody (17859-1-AP)

    IHC staining of human small intestine using 17859-1-AP (same clone as 17859-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine using 17859-1-AP (GCS1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17859-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using GCS1 Polyclonal antibody (17859-1-AP)

    IHC staining of human small intestine using 17859-1-AP (same clone as 17859-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine using 17859-1-AP (GCS1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17859-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using GCS1 Polyclonal antibody (17859-1-AP)

    IHC staining of human small intestine using 17859-1-AP (same clone as 17859-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine using 17859-1-AP (GCS1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17859-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using GCS1 Polyclonal antibody (17859-1-AP)

    IHC staining of human small intestine using 17859-1-AP (same clone as 17859-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine using 17859-1-AP (GCS1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17859-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using GCS1 Polyclonal antibody (17859-1-AP)

    IHC staining of human liver using 17859-1-AP (same clone as 17859-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 17859-1-AP (GCS1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17859-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using GCS1 Polyclonal antibody (17859-1-AP)

    IHC staining of human liver using 17859-1-AP (same clone as 17859-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 17859-1-AP (GCS1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17859-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

GCS1 Polyclonal antibody, PBS Only

GCS1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 17859-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17859-1-PBS targets GCS1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GCS1 fusion protein Ag12321 相同性解析による交差性が予測される生物種
Full Name mannosyl-oligosaccharide glucosidase
Calculated molecular weight 837 aa, 92 kDa
Observed molecular weight 80-92 kDa
GenBank accession numberBC028337
Gene Symbol GCS1
Gene ID (NCBI) 7841
RRIDAB_10694270
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13724
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MOGS (Mannosyl-oligosaccharide glucosidase), also known as GCS1, is the first enzyme in the N-linked oligosaccharide processing pathway. The enzyme cleaves the distal alpha-1,2-linked glucose residue from the Glc(3)-Man(9)-GlcNAc(2) oligosaccharide precursor (PMID: 38187061). The resulting Glc(2)-Man(9)-GlcNAc(2) intermediate is then further modified by glucosidase II and several ER- and Golgi-resident mannosidases and glycosyltransferases, finally yielding a complex array of glycan structures (PMID: 3896128). MOGS has two isoforms, and it is located in the lumen of the endoplasmic reticulum.