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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GCSH Recombinant antibody, PBS Only

GCSH Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230505A3

Cat no : 83012-5-PBS

Synonyms

GCE, GCSH, NKH

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using GCSH Recombinant antibody (83012-5-RR)

    WB analysis of HEK-293 using 83012-5-RR (same clone as 83012-5-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 83012-5-RR (GCSH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83012-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GCSH Recombinant antibody (83012-5-RR)

    WB analysis using 83012-5-RR (same clone as 83012-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83012-5-RR (GCSH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83012-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83012-5-PBS

    Affinity and Kinetic Characterization of 83012-5-PBS

    Biolayer interferometry (BLl) kinetic assays of 83012-5-RR against Human GCSH were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83012-5-PBS is GCSH Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GCSH fusion protein Ag10174 相同性解析による交差性が予測される生物種
Full Name glycine cleavage system protein H (aminomethyl carrier)
Calculated molecular weight 19 kDa
GenBank accession numberBC000790
Gene symbol GCSH
Gene ID (NCBI) 2653
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.