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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GCSH Recombinant antibody

GCSH Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230505A3

Cat no : 83012-5-RR

Synonyms

GCE, GCSH, NKH

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using GCSH Recombinant antibody (83012-5-RR)

    WB analysis of HEK-293 using 83012-5-RR

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 83012-5-RR (GCSH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GCSH Recombinant antibody (83012-5-RR)

    WB analysis using 83012-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83012-5-RR (GCSH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83012-5-RR

    Affinity and Kinetic Characterization of 83012-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83012-5-RR against Human GCSH were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83012-5-RR is GCSH Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GCSH fusion protein Ag10174 相同性解析による交差性が予測される生物種
Full Name glycine cleavage system protein H (aminomethyl carrier)
Calculated molecular weight 19 kDa
Observed molecular weight~15 kDa
GenBank accession numberBC000790
Gene symbol GCSH
Gene ID (NCBI) 2653
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GCSH(Glycine cleavage system H protein, mitochondrial) is a component of the glycine cleavage system loosely associated with the mitochondrial inner membrane and has lipoic acid as a prosthetic group. The full-length GCSH cDNA encodes a precursor protein of 173 amino acids and a mature protein of 125 amino acids. The lipoylation of H-protein occurs in mitochondria which probably contain an activated form of lipoic acid as well as other components required for the transfer of lipoic acid to the protein(PMID:2211640). Defects in GCSH are a cause of non-ketotic hyperglycinemia (NKH).

Protocols

Product Specific Protocols
WB protocol for GCSH antibody 83012-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols