CoraLite® Plus 488-conjugated GDI1 Monoclonal antibody
GDI1 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
4E1G1
Cat no : CL488-66434
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-66434 targets GDI1 in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | GDI1 fusion protein Ag17511 相同性解析による交差性が予測される生物種 |
| Full Name | GDP dissociation inhibitor 1 |
| Calculated molecular weight | 51 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC000317 |
| Gene symbol | GDI1 |
| Gene ID (NCBI) | 2664 |
| RRID | AB_2883336 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family. GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI1 is expressed primarily in neural and sensory tissues and also in secretory cells, displaying a diffuse, cytoplasmic distribution in cells. It runs as a 55 kDa protein in SDS-PAGE. (PMID: 7929030,PMID: 19570034)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 GDI1 antibody CL488-66434 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
