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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated GEF-H1 Polyclonal antibody

GEF-H1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-24472

Synonyms

ARHGEF2, LFP40, KIAA0651, GEFH1, GEF H1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated GEF-H1 Polyclonal an (CL488-24472)

    IF Staining of HepG2 using CL488-24472

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 GEF-H1 antibody (CL488-24472) at dilution of 1:50.

  • Flow cytometry (FC) experiment of HEK-293T cells using CoraLite® Plus 488-conjugated GEF-H1 Polyclonal an (CL488-24472)

    FC experiment of HEK-293T using CL488-24472

    1x10^6 HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated GEF-H1 Polyclonal antibody (CL488-24472)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-24472 targets GEF-H1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GEF-H1 fusion protein Ag13352 相同性解析による交差性が予測される生物種
Full Name rho/rac guanine nucleotide exchange factor (GEF) 2
Calculated molecular weight 112 kDa
Observed molecular weight 111 kDa
GenBank accession numberBC020567
Gene symbol GEF-H1/ARHGEF2
Gene ID (NCBI) 9181
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 GEF-H1 antibody CL488-24472Download protocol
FC protocol for CL Plus 488 GEF-H1 antibody CL488-24472Download protocol
Standard Protocols
Click here to view our Standard Protocols