Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 1 publications below |
Product Information
24897-1-AP targets GEMIN5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GEMIN5 fusion protein Ag19268 相同性解析による交差性が予測される生物種 |
| Full Name | gem (nuclear organelle) associated protein 5 |
| Calculated molecular weight | 1508 aa, 169 kDa |
| Observed molecular weight | 168 kDa |
| GenBank accession number | BC113614 |
| Gene Symbol | GEMIN5 |
| Gene ID (NCBI) | 25929 |
| RRID | AB_2879784 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TEQ6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GEMIN5 also named as Gem associated protein 5 is a 1508 amino-acid protein, which belongs to the WD repeat gemin-5 family. The molecular weight of GEMIN5 is 168 kDa GEMIN5 is a component of SMN complex, which plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. GEMIN5 is identified as the factor that allows the SMN complex to distinguish snRNAs from other cellular RNAs for snRNP biogenesis. GEMIN5 is found in nucleoplasm, nuclear bodies and cytoplasm.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GEMIN5 antibody 24897-1-AP | Download protocol |
| IHC protocol for GEMIN5 antibody 24897-1-AP | Download protocol |
| IF protocol for GEMIN5 antibody 24897-1-AP | Download protocol |
| IP protocol for GEMIN5 antibody 24897-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Dev Cell DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice | ||
Front Cell Dev Biol Autosomal Recessive Cerebellar Atrophy and Spastic Ataxia in Patients With Pathogenic Biallelic Variants in GEMIN5. | ||