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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated GEMIN5 Polyclonal antibody

GEMIN5 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-24897

Synonyms

DKFZp586M1824, Gem associated protein 5, GEMIN5

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated GEMIN5 Polyclonal an (CL488-24897)

    IF Staining of HeLa using CL488-24897

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 GEMIN5 antibody (CL488-24897) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-24897 targets GEMIN5 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GEMIN5 fusion protein Ag19268 相同性解析による交差性が予測される生物種
Full Name gem (nuclear organelle) associated protein 5
Calculated molecular weight 1508 aa, 169 kDa
Observed molecular weight 168 kDa
GenBank accession numberBC113614
Gene symbol GEMIN5
Gene ID (NCBI) 25929
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GEMIN5 also named as Gem associated protein 5 is a 1508 amino-acid protein, which belongs to the WD repeat gemin-5 family. The molecular weight of GEMIN5 is 168 kDa GEMIN5 is a component of SMN complex, which plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. GEMIN5 is identified as the factor that allows the SMN complex to distinguish snRNAs from other cellular RNAs for snRNP biogenesis. GEMIN5 is found in nucleoplasm, nuclear bodies and cytoplasm.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 GEMIN5 antibody CL488-24897Download protocol
Standard Protocols
Click here to view our Standard Protocols