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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GEMIN8 Monoclonal antibody

GEMIN8 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A6B4

Cat no : 67871-1-Ig

Synonyms

Gemin-8, Gemin 8, Gem-associated protein 8, FAM51A1, 1A6B4

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GEMIN8 Monoclonal antibody (67871-1-Ig)

    WB analysis of HeLa using 67871-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67871-1-Ig (GEMIN8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using GEMIN8 Monoclonal antibody (67871-1-Ig)

    WB analysis of NIH/3T3 using 67871-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67871-1-Ig (GEMIN8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GEMIN8 Monoclonal antibody (67871-1-Ig)

    IHC staining of mouse brain using 67871-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67871-1-Ig (GEMIN8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GEMIN8 Monoclonal antibody (67871-1-Ig)

    IF Staining of HepG2 using 67871-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GEMIN8 antibody (67871-1-Ig, Clone: 1A6B4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). DAPI (Blue).

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Tested Applications

Positive WB detected inNIH/3T3 cells, HeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67871-1-Ig targets GEMIN8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GEMIN8 fusion protein Ag6774 相同性解析による交差性が予測される生物種
Full Name gem (nuclear organelle) associated protein 8
Calculated molecular weight 29 kDa
GenBank accession numberBC003607
Gene symbol GEMIN8
Gene ID (NCBI) 54960
RRIDAB_2918629
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GEMIN8, also named as FAM51A1 and Gemin-8, is associated with the SMN complex and is localized in the cytoplasm and in the nucleus, where it is highly concentrated in Gems. Gemin6, Gemin7, and Unrip form a stable cytoplasmic complex whose association with SMN requires Gemin8. Gemin8 binds directly to SMN and mediates its interaction with the Gemin6/Gemin7 heterodimer. This antibody recognize GEMIN8(28-32kd) only.

Protocols

Product Specific Protocols
WB protocol for GEMIN8 antibody 67871-1-IgDownload protocol
IHC protocol for GEMIN8 antibody 67871-1-IgDownload protocol
IF protocol for GEMIN8 antibody 67871-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols