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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GEMIN8 Monoclonal antibody, PBS Only

GEMIN8 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A6B4

Cat no : 67871-1-PBS

Synonyms

Gemin-8, Gemin 8, Gem-associated protein 8, FAM51A1, 1A6B4

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GEMIN8 Monoclonal antibody (67871-1-Ig)

    WB analysis of HeLa using 67871-1-Ig (same clone as 67871-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67871-1-Ig (GEMIN8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67871-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using GEMIN8 Monoclonal antibody (67871-1-Ig)

    WB analysis of NIH/3T3 using 67871-1-Ig (same clone as 67871-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67871-1-Ig (GEMIN8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67871-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GEMIN8 Monoclonal antibody (67871-1-Ig)

    IHC staining of mouse brain using 67871-1-Ig (same clone as 67871-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67871-1-Ig (GEMIN8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67871-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GEMIN8 Monoclonal antibody (67871-1-Ig)

    IF Staining of HepG2 using 67871-1-Ig (same clone as 67871-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GEMIN8 antibody (67871-1-Ig, Clone: 1A6B4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). DAPI (Blue). This data was developed using the same antibody clone with 67871-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67871-1-PBS targets GEMIN8 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GEMIN8 fusion protein Ag6774 相同性解析による交差性が予測される生物種
Full Name gem (nuclear organelle) associated protein 8
Calculated molecular weight 29 kDa
GenBank accession numberBC003607
Gene symbol GEMIN8
Gene ID (NCBI) 54960
RRIDAB_2918629
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GEMIN8, also named as FAM51A1 and Gemin-8, is associated with the SMN complex and is localized in the cytoplasm and in the nucleus, where it is highly concentrated in Gems. Gemin6, Gemin7, and Unrip form a stable cytoplasmic complex whose association with SMN requires Gemin8. Gemin8 binds directly to SMN and mediates its interaction with the Gemin6/Gemin7 heterodimer. This antibody recognize GEMIN8(28-32kd) only.