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Validation Data Gallery

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  • Western Blot (WB) analysis of U2OS cells using GEN1 Monoclonal antibody (68060-1-Ig)

    WB analysis of U2OS using 68060-1-Ig (same clone as 68060-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 68060-1-Ig (GEN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68060-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using GEN1 Monoclonal antibody (68060-1-Ig)

    WB analysis of A549 using 68060-1-Ig (same clone as 68060-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 68060-1-Ig (GEN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68060-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GEN1 Monoclonal antibody (68060-1-Ig)

    WB analysis using 68060-1-Ig (same clone as 68060-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68060-1-Ig (GEN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68060-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using GEN1 Monoclonal antibody (68060-1-Ig)

    WB analysis of A549 using 68060-1-Ig (same clone as 68060-1-PBS)

    WB result of GEN1 antibody (68060-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-GEN1 transfected A549 cells. This data was developed using the same antibody clone with 68060-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

GEN1 Monoclonal antibody, PBS Only

GEN1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68060-1-PBS
Clone No.1A10D10

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Gen, 1A10D10

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68060-1-PBS targets GEN1 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GEN1 fusion protein Ag31247 相同性解析による交差性が予測される生物種
Full Name Gen homolog 1, endonuclease (Drosophila)
Calculated molecular weight 103 kDa
Observed molecular weight98-100 kDa
GenBank accession numberNM_182625
Gene Symbol GEN1
Gene ID (NCBI) 348654
RRIDAB_2918801
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ17RS7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GEN1 is a endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. GEN1 efficiently cleaves both single and double HJs contained within large recombination intermediates.