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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GEN1 Monoclonal antibody

GEN1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A10D10

Cat no : 68060-1-Ig

Synonyms

DKFZp781F0986, FLJ40869, Gen, GEN1

Validation Data Gallery

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  • Western Blot (WB) analysis of U2OS cells using GEN1 Monoclonal antibody (68060-1-Ig)

    WB analysis of U2OS using 68060-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 68060-1-Ig (GEN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using GEN1 Monoclonal antibody (68060-1-Ig)

    WB analysis of A549 using 68060-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 68060-1-Ig (GEN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GEN1 Monoclonal antibody (68060-1-Ig)

    WB analysis using 68060-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68060-1-Ig (GEN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHEK-293 cells, U2OS cells, A549 cells, K-562 cells, Daudi cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68060-1-Ig targets GEN1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GEN1 fusion protein Ag31247 相同性解析による交差性が予測される生物種
Full Name Gen homolog 1, endonuclease (Drosophila)
Calculated molecular weight 103 kDa
Observed molecular weight98-100 kDa
GenBank accession numberNM_182625
Gene symbol GEN1
Gene ID (NCBI) 348654
RRIDAB_2918801
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

GEN1 is a endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. GEN1 efficiently cleaves both single and double HJs contained within large recombination intermediates.

Protocols

Product Specific Protocols
WB protocol for GEN1 antibody 68060-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols